Publications related to Western blot

Bubble cell for magnetic bead trapping in capillary electrophoresis

A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case wi ...

Springer Verlag2011

Microchannel-guided capture antibody patterning on beads for multiple protein detection arrays

Martinus Gijs, Hüseyin Cumhur Tekin, Venkataragavalu Sivagnanam, Sébastien Vaneberg

We present the rapid fabrication of custom protein micro-arrays, in less than 35 minutes, on the surface of immobilized beads using microchannel-guided flows driven by a single syringe pump. The commercially available beads provide robust micro-array surfa ...

2011

Beta amyloid and hyperphosphorylated tau deposits in the pancreas in type 2 diabetes

László Forró, Aleksandra Radenovic, Andras Kis

Strong epidemiologic evidence suggests an association between Alzheimer disease (AD) and type 2 diabetes. To determine if amyloid beta (A

\beta

) and hyperphosphorylated tau occurs in type 2 diabetes, pancreas tissues from 21 autopsy cases (10 type 2 diabe ...

Elsevier2010

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain

Oliver Hantschel

Interactions between Src homology 2 (SH2) domains and phosphotyrosine sites regulate tyrosine kinase signaling networks. Selective perturbation of these interactions is challenging due to the high homology among the 120 human SH2 domains. Using an improved ...

2010

Nonfouling Polypeptide Brushes via Surface-initiated Polymerization of N-epsilon-oligo(ethylene glycol)succinate-L-lysine N-carboxyanhydride

Jian Wang, Harm-Anton Klok, Matthew Gibson, Raphaël Barbey

This contribution describes the preparation of nonfouling polypeptide brushes via surface-initiated ring-opening polymerization of oligo(ethylene glycol) modified L-lysine N-carboxyanhydrides. Circular dichroism experiments indicated that these surface-anc ...

2009

Hybrid Control and Transport Using Bacteria-Driven Microbiorobots

Mahmut Selman Sakar

We are examining techniques for manipulation of microfabricated elements using arrays of bacteria as microactuators. Flagellated Serratia marcescens bacteria are attached to microstructures using a blotting technique that creates a bacterial monolayer carp ...

ASME2009

Magnetic beads retention device for sandwich immunoassay: comparison of off-chip and on-chip antibody incubation

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme

We use magnetic microbeads, which are magnetically self-assembled in chains in a microfluidic chip, as reaction substrates to implement two different sandwich immunoassay protocols for the detection of mouse monoclonal target antibodies. The magnetic chain ...

Springer-Verlag2009

Harnessing bacterial power in microscale actuation

Mahmut Selman Sakar

This paper presents a systematic analysis of the motion of microscale structures actuated by flagellated bacteria. We perform the study both experimentally and theoretically. We use a blotting procedure to attach flagellated bacteria to a buoyancy-neutral ...

IEEE2009

Differential regulation of Dlg1, Scrib, and Lgl1 expression in a transgenic mouse model of ocular cancer

Friedrich Beermann

Purpose: Discs large (dlg), scribble (scrib), and lethal giant larvae (lgl) are major suppressor genes in Drosophila melanogaster. They encode proteins that regulate cell polarity and cell proliferation in Drosophila and mammals. However, their basic oncog ...

2008

The human SPT20-containing SAGA complex plays a direct role in the regulation of endoplasmic reticulum stress-induced genes

One of the central questions in eukaryotic transcription is how activators can transmit their signal to stimulate gene expression in the context of chromatin. The multisubunit SAGA coactivator complex has both histone acetyltransferase and deubiquitination ...

2008

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Bubble cell for magnetic bead trapping in capillary electrophoresis

Microchannel-guided capture antibody patterning on beads for multiple protein detection arrays

Beta amyloid and hyperphosphorylated tau deposits in the pancreas in type 2 diabetes

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain

Nonfouling Polypeptide Brushes via Surface-initiated Polymerization of N-epsilon-oligo(ethylene glycol)succinate-L-lysine N-carboxyanhydride

Hybrid Control and Transport Using Bacteria-Driven Microbiorobots

Magnetic beads retention device for sandwich immunoassay: comparison of off-chip and on-chip antibody incubation

Harnessing bacterial power in microscale actuation

Differential regulation of Dlg1, Scrib, and Lgl1 expression in a transgenic mouse model of ocular cancer

The human SPT20-containing SAGA complex plays a direct role in the regulation of endoplasmic reticulum stress-induced genes

Differential regulation of Dlg1, Scrib, and Lgl1 expression in a transgenic mouse model of ocular cancer

The human SPT20-containing SAGA complex plays a direct role in the regulation of endoplasmic reticulum stress-induced genes

Nonfouling Polypeptide Brushes via Surface-initiated Polymerization of N-epsilon-oligo(ethylene glycol)succinate-L-lysine N-carboxyanhydride

Beta amyloid and hyperphosphorylated tau deposits in the pancreas in type 2 diabetes

Hybrid Control and Transport Using Bacteria-Driven Microbiorobots

Magnetic beads retention device for sandwich immunoassay: comparison of off-chip and on-chip antibody incubation

Harnessing bacterial power in microscale actuation

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain

Bubble cell for magnetic bead trapping in capillary electrophoresis

Microchannel-guided capture antibody patterning on beads for multiple protein detection arrays