Concept

Digital microscope

Related publications (71)

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Multi-plane super-resolution microscopy

Azat Sharipov

Understanding cell functions is the major goal of molecular biology, which intends to elucidate the interactions between biomolecules at a subcellular level. One of the widely used techniques in molecular biology is fluorescence microscopy, which offers hi ...

EPFL2017

Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

Suliana Manley, Kyle Michael Douglass, Christian Sieben, Anna Archetti, Ambroise Justin Lambert

Biological processes are inherently multi-scale, and supramolecular complexes at the nanoscale determine changes at the cellular scale and beyond. Single-molecule localizationmicroscopy (SMLM)(1-3) techniques have been established as important tools for st ...

Nature Publishing Group2016

Intensity-Based Point-Spread-Function-Aware Registration for Multi-View Applications in Optical Microscopy

Michael Stefan Daniel Liebling

We present an algorithm to spatially register two volumetric datasets related via a rigid-body transform and degraded by an anisotropic point-spread-function (PSF). Registration is necessary, for example, when fusing data in multi-view microscopy. Automati ...

2015

Broadband Digital Holographic Camera for Microscopy

Zahra Monemhaghdoust

In this thesis, we explore a combination of analog and digital holographic techniques to demonstrate high speed quantitative phase imaging (QPI) using low coherence (broad bandwidth) light sources. Off-axis digital holographic microscopy is inherently a co ...

EPFL2015

Concept of a multichannel spin-resolving electron analyzer based on Mott scattering

Jan Hugo Dil, Vladimir N. Strocov

The concept of a multichannel electron spin detector based on optical imaging principles and Mott scattering (iMott) is presented. A multichannel electron image produced by a standard angle-resolving (photo) electron analyzer or microscope is re-imaged by ...

International Union of Crystallography2015

CIDRE: an illumination-correction method for optical microscopy

Kevin Smith, Yunpeng Li

Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do ...

Nature Publishing Group2015

Portable advanced off-axis camera for quantitative phase microscopy

Christian Depeursinge

We propose and experimentally demonstrate a device in which common-path interferometry combined with off-axis holographic geometry is used to realize a digital holographic camera which can be attached to the camera port of a conventional transmission micro ...

Spie-Int Soc Optical Engineering2014

Optical microscopy for biology and life sciences

Theo Lasser, Marcel Leutenegger

Optical microscopy has become a major non- or minimally invasive tool for investigating living organisms such as cells, tissues or small animals. Being such an essential tool for research and because lacking knowledge and insight in the working principles ...

Laboratoire d'Optique Biomédicale, EPFL2014

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A large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parame ...

Royal Soc Chemistry2014

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In Digital holography Microscopes (DHM) implemented in the so-called "off axis" configuration, the object and reference wave fronts are not co-planar but form an angle of a few degrees. This results into two main drawbacks. First, the contrast of the inter ...

Optical Soc Amer2013