Concept

Two-hybrid screening

Related publications (96)

A yeast one-hybrid and microfluidics-based pipeline to map mammalian gene regulatory networks

Bart Deplancke, Sebastian Martin Waszak, Sunil Kumar Raghav, Alina Isakova, Jovan Simicevic, Jean-Daniel Feuz, Antonina Iagovitina

The comprehensive mapping of gene promoters and enhancers has significantly improved our understanding of how the mammalian regulatory genome is organized. An important challenge is to elucidate how these regulatory elements contribute to gene expression b ...

Nature / European Molecular Biology Organization2013

Hybrid probabilities and error-domain structural identification using ambient vibration monitoring

Ian Smith, Clotaire Michel, James-Alexandre Goulet

For the assessment of structural behaviour, many approaches are available to compare model predictions with measurements. However, few approaches include uncertainties along with dependencies associated with models and observations. In this paper, an error ...

Elsevier2013

The THO complex component Thp2 counteracts telomeric R-loops and telomere shortening

Joachim Lingner, Verena Pfeiffer, Larissa Grolimund, Jérôme Crittin

Telomere maintenance by the conventional DNA replication machinery and telomerase is assisted by specialized DNA helicases, nucleases and telomere binding proteins. Here, we identify the THO components at telomeres and define critical roles of this complex ...

Nature Publishing Group2013

Labelling of Fusion Proteins with Synthetic Probes

Kai Johnsson, Alexandre Juillerat

The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O6-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The substr ...

2013

TiO2 nanoparticles suppress Escherichia coli cell division in the absence of UV irradiation in acidic conditions

Juan Kiwi

TiO2 nanoparticles (NPs) activated by UV irradiation are known to have a bactericidal effect. In this study we report the details of TiO2 NPs influence on the colony-forming capacity of E. coli in the dark at pH 4.0-4.5. At this pH the bacterial cells are ...

2012

Characterization of semisynthetic and naturally Nα-acetylated α-synuclein in vitro and in intact cells: implications for aggregation and cellular properties of α-synuclein

Hilal Lashuel, Bruno Claude Daniel Fauvet, Mohamed Bilal Fares

N-terminal acetylation is a very common post-translational modification, although its role in regulating protein physical properties and function remains poorly understood. α-Synuclein (α-syn), a protein that has been linked to the pathogenesis of Parkinso ...

Amer Soc Biochemistry Molecular Biology Inc2012

Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

Philippe Renaud, Freddy Radtke, Rajwinder Lehal, Rodrigo Martinez Duarte

Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (108 yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of ...

2012

Rapid Synthesis of Defined Eukaryotic Promoter Libraries

Sebastian Maerkl, Arun Stephen Rajkumar

Current gene synthesis methods allow the generation of long segments of dsDNA. We show that these techniques can be used to create synthetic regulatory elements and describe a method for the creation of completely defined, synthetic variants of the PHOS pr ...

Amer Chemical Soc2012

Room-temperature combinatorial screening of cyclometallated iridium(III) complexes for a step towards molecular control of colour purity

Michael Graetzel, Mohammad Khaja Nazeeruddin, Rosario Scopelliti, Euro Solari, Il Jung

A library of emission spectra of 90 bis-cyclometallated iridium complexes has been obtained using a simple combinatorial approach performed at room temperature. Trends in emission maxima are rationalized using Hammett parameters and invoking inter ligand e ...

Royal Society of Chemistry2011

Redundancy and specificity of multiple Trigger Factor chaperones in Desulfitobacteria

Christof Holliger, Julien Maillard

The ribosome-bound Trigger Factor (TF) chaperone assists folding of newly synthesized polypeptides and participates in the assembly of macromolecular complexes. In the present study we showed that multiple distinct TF paralogues are present in genomes of D ...

2011

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