Concept

Two-photon excitation microscopy

Related publications (489)

Multi-plane super-resolution microscopy

Understanding cell functions is the major goal of molecular biology, which intends to elucidate the interactions between biomolecules at a subcellular level. One of the widely used techniques in molecular biology is fluorescence microscopy, which offers hi ...

EPFL2017

Multi-harmonic imaging in the second near-infrared window of nanoparticle-labeled stem cells as effective monitoring tool in tissue depth

Sandrine Gerber, Solène Marcelle Françoise Marie Passemard, Luigi Bonacina

In order to assess the therapeutic potential of cell-based strategies, it is of paramount importance to elaborate and validate new tools for monitoring the behavior of injected cells in terms of tissue dissemination and engraftment properties. Here, we app ...

American Chemical Society2017

Microscopy and digital light shaping through optical fibers

Damien Claude-Marie Loterie

Microscopy is an essential tool in medicine and biomedical research. Traditional microscopes rely on bulky optics, complicating their usage when studying live animal tissues. In addition, light cannot penetrate very far in most biological tissues due to sc ...

EPFL2017

Formation of Large Hypericin Aggregates in Giant Unilamellar Vesicles-Experiments and Modeling

Jaroslava Joniová, Veronika Huntosova

The incorporation of hypericin (Hyp) from aqueous solutions into giant unilamellar vesicle (GUV) membranes has been studied experimentally and by means of kinetic Monte Carlo modeling. The time evolution of Hyp fluorescence originating from Hyp monomers di ...

Cell Press2017

Damage recovery after impact in E-glass reinforced poly(epsilon-caprolactone)/epoxy blends

Véronique Michaud, Amaël Maximilien Cohades

Damage recovery after low-velocity impact has been assessed in woven E-glass fibre-reinforced polymer composites with an epoxy matrix and a blend of epoxy and 25 vol% of poly(e-caprolactone) (PCL). Impact was carried out at three energy levels (8.5, 17, 34 ...

Elsevier2017

Harmonium: An Ultrafast Vacuum Ultraviolet Facility

Majed Chergui, Marco Grioni, Marcel Drabbels, Frank van Mourik, Alberto Crepaldi, Christopher Alexander Arrell, Gianmarco Gatti, José Javier Pancracio Ojeda Andara, Andrew Daniel Bonnar Clark, Luca Longetti, Silvan Dino Marcos Roth

Harmonium is a vacuum ultraviolet (VUV) photon source built within the Lausanne Centre for Ultrafast Science (LACUS). Utilising high harmonic generation, photons from 20-110 eV are available to conduct steady-state or ultrafast photoelectron and photoion s ...

Swiss Chemical Soc2017

Combining PALM and SOFI for quantitative imaging of focal adhesions in living cells

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Tomas Lukes, Hendrik Georges Deschout, Azat Sharipov

Focal adhesions are complicated assemblies of hundreds of proteins that allow cells to sense their extracellular matrix and adhere to it. Although most focal adhesion proteins have been identified, their spatial organization in living cells remains challen ...

Spie-Int Soc Optical Engineering2017

Single photons from dissipation in coupled cavities

Vincenzo Savona, Hugo Pierre Alexandre Flayac

We propose a single-photon source based on a pair of weakly nonlinear optical cavities subject to a one-directional dissipative coupling. When both cavities are driven by mutually coherent fields, sub-Poissonian light is generated in the target cavity even ...

Amer Physical Soc2016

Wide-field medium-repetition-rate multiphoton microscopy reduces photodamage of living cells

Sylvie Roke, Carlos Alberto Macias Romero, Siyuan Wang, Vitalijs Zubkovs

Demands of higher spatial and temporal resolutions in linear and nonlinear imaging keep pushing the limits of optical microscopy. We showed recently that a multiphoton microscope with 200 kHz repetition rate and wide-field illumination has a 2–3 orders of ...

Optical Soc Amer2016

Correlating microscopy techniques and ToF-SIMS analysis of fully grown mammalian oocytes

Sami Rtimi

The 2D-molecular thin film analysis protocol for fully grown mice oocytes is described using an innovative approach. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and optical mi ...

Royal Soc Chemistry2016

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