Concept
Provirus
Related publications (24)
Direct and Retrograde Transduction of Nigral Neurons with AAV6, 8, and 9 and Intraneuronal Persistence of Viral Particles
Patrick Aebischer, Bernard Schneider
Recombinant adeno-associated viral (AAV) vectors of serotypes 6, 8, and 9 were characterized as tools for gene delivery to dopaminergic neurons in the substantia nigra for future gene therapeutic applications in Parkinson's disease. While vectors of all th ...
Mary Ann Liebert2013TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells
Didier Trono, Adamantia Kapopoulou, Andrea Corsinotti, Helen Mary Rowe, Johan Jakobsson
TRIM28 is critical for the silencing of endogenous retroviruses (ERVs) in embryonic stem (ES) cells. Here, we reveal that an essential impact of this process is the protection of cellular gene expression in early embryos from perturbation by cis-acting act ...
2013Embryonic stem cell potency fluctuates with endogenous retrovirus activity
Embryonic stem (ES) cells are derived from blastocyst-stage embryos and are thought to be functionally equivalent to the inner cell mass, which lacks the ability to produce all extraembryonic tissues. Here we identify a rare transient cell population withi ...
2012Profaning the Ultimate Sanctuary: HIV Latency in Hematopoietic Stem Cells
The early establishment of a reservoir of latently infected T cells is a sobering obstacle to HIV eradication, in spite of the efficacy of current antiretroviral therapies. That latent proviruses might also hide in multipotent hematopoietic stem cells sugg ...
2011Analysis of HIV-1 Expression Level and Sense of Transcription by High-Throughput Sequencing of the Infected Cell
Jacques Rougemont, Amalio Telenti
Next-generation sequencing offers an unprecedented opportunity to jointly analyze cellular and viral transcriptional activity without prerequisite knowledge of the nature of the transcripts. SupT1 cells were infected with a vesicular stomatitis virus G env ...
2011Dynamic control of endogenous retroviruses during development
Close to half of the human genome encompasses mobile genetic elements, most of which are retrotransposons. These genetic invaders are formidable evolutionary forces that have shaped the architecture of the genomes of higher organisms, with some conserving ...
Elsevier2011KAP1 controls endogenous retroviruses in embryonic stem cells
Didier Trono, Daniel Constam, Jacques Rougemont, Sonia Verp, Séverine Reynard, Helen Mary Rowe, Johan Jakobsson, Pierre Maillard, Hillary Layard-Liesching
More than forty per cent of the mammalian genome is derived from retroelements, of which about one-quarter are endogenous retroviruses (ERVs). Some are still active, notably in mice the highly polymorphic early transposon (ETn)/MusD and intracisternal A-ty ...
Nature Publishing Group2010Studies on lentiviral-mediated transgenesis
Transgenic animals are essential research tools, whether to address basic biological questions or to develop preclinical models of human diseases. Their generation through the injection of naked plasmid DNA into the male pronucleus of a fertilized oocyte h ...
EPFL2009Entry and transcription as key determinants of differences in CD4 T-cell permissiveness to human immunodeficiency virus type 1 infection
Isolated primary human cells from different donors vary in their permissiveness-the ability of cells to be infected and sustain the replication of human immunodeficiency virus type 1 (HIV-1). We used replicating HIV-1 and single-cycle lentivirus vectors in ...
2004Specific recognition of the viral protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T cells mediates the non-MHC-restricted lysis of human cytomegalovirus-infected cells
Immune evasion mechanisms of human CMV are known; however, the immune control of infection remains poorly elucidated. We show that interaction between the viral protein UL18 on infected cells and the invariant receptor CD85j/LIR-1/ILT2 expressed on CTL is ...
2004