Concept

Cytometry

Related publications (36)

Portable impedance-sensing device for microorganism characterization in the field

A variety of biosensors have been proposed to quickly detect and measure the properties of individual microorganisms among heterogeneous populations, but challenges related to cost, portability, stability, sensitivity, and power consumption limit their app ...

NATURE PORTFOLIO2023

Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting

Christoph Merten, Jatin Panwar, Alexis Louis Autour

Droplet microfluidics has revolutionized quantitative high-throughput bioassays and screening, especially in the field of single-cell analysis where applications include cell characterization, antibody discovery and directed evolution. However, droplet mic ...

NATURE PORTFOLIO2023

Portable Multi-Frequency Impedance-Sensing Device for Bacteria Classification in a Flowing Liquid

Sandro Carrara, Karim Bouzid

This paper presents a novel impedance-sensing device and its related microfluidics system developed to characterize the macroscopical properties of bacteria and microparticles. The system is based on a phase-sensitive detector with square-waves excitation ...

IEEE2022

Simplified Instrument Calibration forWide-FieldFluorescence Resonance Energy Transfer (FRET) Measured by the Sensitized Emission Method

Forster (or fluorescence) resonance energy transfer (FRET) is a quantifiable energy transfer in which a donor fluorophore nonradiatively transfers its excitation energy to an acceptor fluorophore. A change in FRET efficiency indicates a change of proximity ...

2020

Impedance-Based Single-Cell Pipetting

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Jean-Baptiste Bureau, Georges Muller, Nicolas Jean Bernard Beuchat, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here ...

SAGE PUBLICATIONS INC2020

Using flow cytometry and light-induced fluorescence to characterize the variability and characteristics of bioaerosols in springtime in Metro Atlanta, Georgia

Athanasios Nenes

The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation, and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large ...

COPERNICUS GESELLSCHAFT MBH2020

How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study

Philippe Renaud, Harald Van Lintel, Jonathan Bastien Cottet

This paper describes a comprehensive analysis of the geometrical parameters in uencing the sensitivity of a coplanar elec- trode layout for electrical impedance ow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3 ...

2019

Rational design of a high-throughput droplet sorter

Tobias Schneider, Simon Sebastian Schütz

The high-throughput selection of individual droplets is an essential function in droplet-based microfluidics. Fluorescence-activated droplet sorting is achieved using electric fields triggered at rates up to 30 kHz, providing the ultra-high throughput rele ...

2019

A Versatile, Low-Cost, Multiway Microfluidic Sorter for Droplets, Cells, and Embryos

Christoph Merten

Partitioning and sorting particles, including molecules, cells and organisms, is an essential prerequisite for a diverse range of applications. Here, we describe a very economical microfluidic platform (built from parts costing about U.S. $6800 for a stand ...

2018

Interference of engineered nanomaterials in flow cytometry: A case study

Aurélie Walter

Nanotechnology is regarded as the enabling technology of the 21st century. However, only a relatively small number of nano-enabled medical and healthcare products finally made their way to the market. There are several reasons why such innovative approache ...

ELSEVIER SCIENCE BV2018

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