Publications associées à Cytométrie

Portable impedance-sensing device for microorganism characterization in the field

A variety of biosensors have been proposed to quickly detect and measure the properties of individual microorganisms among heterogeneous populations, but challenges related to cost, portability, stability, sensitivity, and power consumption limit their app ...

Nature Portfolio2023

Portable Multi-Frequency Impedance-Sensing Device for Bacteria Classification in a Flowing Liquid

Sandro Carrara, Karim Bouzid

This paper presents a novel impedance-sensing device and its related microfluidics system developed to characterize the macroscopical properties of bacteria and microparticles. The system is based on a phase-sensitive detector with square-waves excitation ...

IEEE2022

Simplified Instrument Calibration forWide-FieldFluorescence Resonance Energy Transfer (FRET) Measured by the Sensitized Emission Method

Forster (or fluorescence) resonance energy transfer (FRET) is a quantifiable energy transfer in which a donor fluorophore nonradiatively transfers its excitation energy to an acceptor fluorophore. A change in FRET efficiency indicates a change of proximity ...

2021

Impedance-Based Single-Cell Pipetting

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Jean-Baptiste Bureau, Georges Muller, Nicolas Jean Bernard Beuchat, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here ...

SAGE PUBLICATIONS INC2020

How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study

Philippe Renaud, Jonathan Bastien Cottet, Harald Van Lintel

This paper describes a comprehensive analysis of the geometrical parameters in uencing the sensitivity of a coplanar elec- trode layout for electrical impedance ow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3 ...

2019

Impedance Spectroscopy for Single Cell Analysis and Dispensing

David Vincent Bonzon

Flow cytometry is an essential tool in biology and medicine with applications in immunodeficiency diagnostic, immunosurveillence, organ transplantation in addition to its basic research usage. Before the widespread use of the Fluorescent-Activated Cell Sor ...

EPFL2017

Microfluidic-integrated vertical electrodes employed in impedance-based cytometry : potential application in immunotherapies

Enrica Rollo

During the last decades, the growing interest for single-cell analysis has led to the creation of a number of microfluidic and lab-on-a chip (LOC) platforms for characterizing cellular samples. In that context label-free based platforms are minimally invas ...

EPFL2016

Digital microfluidics for time-resolved cytotoxicity studies on single non-adherent yeast cells

Martinus Gijs, Matteo Cornaglia

Single cell analysis (SCA) has gained increased popularity for elucidating cellular heterogeneity at genomic, proteomic and cellular levels. Flow cytometry is considered as one of the most widely used techniques to characterize single cell responses; howev ...

Royal Soc Chemistry2015

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Gerardo Turcatti, Benjamin Rappaz, Marc Chambon, Jonas Kühn, Etienne Shaffer, Jérôme Parent, Julien Mena

We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screenin ...

Mary Ann Liebert2013

Automated analysis of single stem cells in microfluidic traps

Matthias Lütolf, Alessandra Griffa, Arne Seitz, Olivier Burri, Stefan Kobel, Mukul Girotra

We report a reliable strategy to perform automated image cytometry of single (non-adherent) stem cells captured in microfluidic traps. The method rapidly segments images of an entire microfluidic chip based on the detection of horizontal edges of microflui ...

2012