Publications related to Photobleaching

NIR Fluorescence lifetime macroscopic imaging with a time-gated SPAD camera

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

The performance of SwissSPAD2 (SS2), a large scale, widefield time-gated CMOS SPAD imager developed for fluorescence lifetime imaging, has recently been described in the context of visible range and fluorescence lifetime imaging microscopy (FLIM) of dyes w ...

SPIE-INT SOC OPTICAL ENGINEERING2022

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Michael Stefan Daniel Liebling, Christian Jaques

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

2020

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Edoardo Charbon, Claudio Bruschini, Ming-Lo Wu, Kazuhiro Morimoto

Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a rela ...

NATURE RESEARCH2020

Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

Fluorescence lifetime imaging (FLI) is increasingly recognized as a powerful tool for biochemical and cellular investigations, including in vivo applications. Fluorescence lifetime is an intrinsic characteristic of any fluorescent dye which, to a large ext ...

AMER CHEMICAL SOC2020

Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy

Demetri Psaltis, Navid Borhani

Deep neural networks have been used to map multi-modal, multi-photon microscopy measurements of a label-free tissue sample to its corresponding histologically stained brightfield microscope colour image. It is shown that the extra structural and functional ...

OPTICAL SOC AMER2019

Strategies for increasing the throughput of super-resolution microscopies

Suliana Manley, Dora Mahecic, Juliette Griffie

Fluorescence microscopy methods have been developed to circumvent the diffraction limit by exploiting nonlinearities in the interactions between light and fluorophores. Initially, these methods were up to orders of magnitude slower than standard microscopi ...

ELSEVIER SCI LTD2019

Time-resolved molecule counting by photon statistics across the visible spectrum

Kristin Stefanie Grussmayer

In the past few years quantification of fluorescently labeled (bio-) molecules has become of increasing importance and several approaches have been developed to address this task. Counting by photon statistics measures the distribution of multiple photon d ...

Royal Society of Chemistry2017

Review of the role played by the photosensitizer's photobleaching during photodynamic therapy

Georges Wagnières, Matthieu Philippe Emmanuel Zellweger

Photodynamic therapy (PDT) is based on the combined administration of photosensitizing drugs and light to treat a variety of malignant, pre-malignant and also non-malignant diseases. The reactive species resulting from the excitation of the photosensitizer ...

Natl Inst Optoelectronics2016

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Photobleaching

NIR Fluorescence lifetime macroscopic imaging with a time-gated SPAD camera

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics

Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy

Strategies for increasing the throughput of super-resolution microscopies

Interference of engineered nanomaterials in flow cytometry: A case study

Time-resolved molecule counting by photon statistics across the visible spectrum

Review of the role played by the photosensitizer's photobleaching during photodynamic therapy

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Chat with Graph Search

Interference of engineered nanomaterials in flow cytometry: A case study

NIR Fluorescence lifetime macroscopic imaging with a time-gated SPAD camera

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics

Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy

Review of the role played by the photosensitizer's photobleaching during photodynamic therapy

Strategies for increasing the throughput of super-resolution microscopies

Time-resolved molecule counting by photon statistics across the visible spectrum