Concept
Negative stain
Related publications (32)
A maximum likelihood approach to two-dimensional crystals
Maximum likelihood (ML) processing of transmission electron microscopy images of protein particles can produce reconstructions of superior resolution due to a reduced reference bias. We have investigated a ML processing approach to images centered on the u ...
Elsevier BV2007The Colored Revolution of Bioimaging
Michaël Unser, Cédric René Jean Vonesch, François Aguet
With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...
IEEE2006Un hôpital psychiatrique au XXIe siècle (Région Parisienne, Stains, F)
L'épidémie de dépression qui affecte lourdement nos sociétés occidentales est le point de départ d'une démocratisation et d'une dédramatisation de la psychiatrie. Face à l'évolution des pratiques médicales et sociales, le caractère de l'hôpital psychiatriq ...
2005Imaging cells and tissues with refractive index radiology
Giorgio Margaritondo, Amela Groso, Yong Yang
Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissue ...
2004Extended Depth-of-Field for Color Images in Light Microscopy: Image Fusion and 3D Visualization
Michaël Unser, Dimitri Nestor Alice Van De Ville, Niels Quack, Daniel Sage
Bright-field microscopy suffers from a relatively small depth-of-field. Typically, the specimen’s profile covers a range larger than the depth-of-field, and parts of the specimen that lie outside the object plane appear blurred. The specimen can be ‘scanne ...
2004The 3.7 angstrom projection map of the glycerol facilitator GlpF: a variant of the aquaporin tetramer
Henning Paul-Julius Stahlberg, Andreas Engel
GlpF, the glycerol facilitator protein of Escherichia coli, is an archetypal member of the aquaporin superfamily. To assess its structure, recombinant histidine-tagged protein was overexpressed, solubilized in octylglucoside and purified to homogeneity. Ne ...
Wiley-Blackwell2000Immunohistochemistry for the MEPHISTO X-PEEM
Over almost 50 years of its development, the science of immunology has become an indispensable tool for the understanding of the histology of tissues. Antibodies are highly specific probes, so that tissue structure can be interpreted not just by morphologi ...
Amer Inst Physics2000The reaction center complex from the green sulfur bacterium Chlorobium tepidum: A structural analysis by scanning transmission electron microscopy
Henning Paul-Julius Stahlberg, Andreas Engel
The three-dimensional (3D) structure of the reaction center (RC) complex isolated from the green sulfur bacterium Chlorobium tepidum was determined from projections of negatively stained preparations by angular reconstitution. The purified complex containe ...
Elsevier BV1999Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation
Florian Maria Wurm, Martin Jordan
DNA-calcium phosphate co-precipitates arise spontaneously in supersaturated solutions. Highly effective precipitates for transfection purposes, however, can be generated only in a very narrow range of physico-chemical conditions that control the initiation ...
Oxford University Press1996High-resolution holography and biopsy: Preliminary results
For holographic biopsy purposes, holograms of biological tissue samples have been recorded in vitro to establish the possibility of differentiating tissues on the holograms. With the use of specific absorbing dyes for staining tissues, the contrast has bee ...
1995