Concept
Staining
Related publications (124)
Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment
Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü, Yichen Feng
Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...
Spie-Soc Photo-Optical Instrumentation Engineers2024Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes
Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures i ...
2023A general strategy to develop fluorogenic polymethine dyes for bioimaging
Pablo Rivera Fuentes, Annabell Martin
Fluorescence imaging is an invaluable tool to study biological processes and further progress depends on the development of advanced fluorogenic probes that reach intracellular targets and label them with high specificity. Excellent fluorogenic rhodamine d ...
Berlin2023Urtica dioica Leaf Infusion Enhances the Sensitivity of Triple-Negative Breast Cancer Cells to Cisplatin Treatment
Urtica dioica (UD) has been widely used in traditional medicine due to its therapeutic benefits, including its anticancer effects. Natural compounds have a promising potential when used in combination with chemotherapeutic drugs. The present study explores ...
MDPI2023Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
Sandor Kasas, María Inés Villalba, Allan Bonvallat, Eugenia Rossetti
Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is ch ...
NATL ACAD SCIENCES2023Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry
Demetri Psaltis, Joowon Lim, Daniele Pirone
The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. Quantitative ph ...
NATURE PORTFOLIO2022Near-Infrared Plasmon-Induced Hot Electron Extraction Evidence in an Indium Tin Oxide Nanoparticle/Monolayer Molybdenum Disulfide Heterostructure
In this work, we observe plasmon-induced hot electron extraction in a heterojunction between indium tin oxide nanocrystals and monolayer molybdenum disulfide. We study the sample with ultrafast differential transmission, exciting the sample at 1750 nm wher ...
AMER CHEMICAL SOC2022Deep Learning Enables Individual Xenograft Cell Classification in Histological Images by Analysis of Contextual Features
Michaël Unser, Cathrin Brisken, Daniel Sage, Olivier Burri, Martin Weigert, Fabio De Martino, Quentin Juppet
Patient-Derived Xenografts (PDXs) are the preclinical models which best recapitulate inter- and intra-patient complexity of human breast malignancies, and are also emerging as useful tools to study the normal breast epithelium. However, data analysis gener ...
2021Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence-Based Microfluidic Diffusion Sizing
Pablo Rivera Fuentes, Adam Miklos Eördögh
Extracellular vesicles (EVs) are emerging as promising diagnostic and therapeutic tools for a variety of diseases. The characterization of EVs requires a series of orthogonal techniques that are overall time- and material-consuming. Here, a microfluidic de ...
WILEY2021Identification of a Dps contamination in Mitomycin-C–induced expression of Colicin Ia
Colicins are bacterial toxins targeting Gram-negative bacteria, including E. coli and related Enterobacteriaceae strains. Some colicins form ion-gated pores in the inner membrane of attacked bacteria that are lethal to their target. Colicin Ia was the firs ...
2021