Publications related to Directed evolution

Phage Selection of Photoswitchable Peptide Ligands

Christian Heinis, Inmaculada Rentero Rebollo, Shiyu Chen, Silvia Bellotto, Hermann A. Wegner

Photoswitchable ligands are powerful tools to control biological processes at high spatial and temporal resolution. Unfortunately, such ligands exist only for a limited number of proteins and their development by rational design is not trivial. We have dev ...

American Chemical Society2014

Fluorescent Labeling of SNAP-Tagged Proteins in Cells

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

One of the most prominent self-labeling tags is SNAP-tag. It is an in vitro evolution product of the human DNA repair protein O 6-alkylguanine-DNA alkyltransferase (hAGT) that reacts specifically with benzylguanine (BG) and benzylchloropyrimidine (CP) deri ...

Springer New York2014

Development of mutagenicity during degradation of N-nitrosamines by advanced oxidation processes

Urs von Gunten, Kristin Schirmer, Silvio Canonica

Development of mutagenicity of five N-nitrosamines (N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosodi-n-propylamine (NDPA), N-nitrosopyrrolidine (NPYR) and N-nitrosodiphenylamine (NDPhA)) was investigated during oxidative processes i ...

Elsevier2014

Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae

Melanie Blokesch, Mirella Lo Scrudato

BackgroundThe human pathogen Vibrio cholerae normally enters the developmental program of natural competence for transformation after colonizing chitinous surfaces. Natural competence is regulated by at least three pathways in this organism: chitin sensing ...

BioMed Central2014

Directed Evolution of Bicyclic Peptides for Therapeutic Application

Christian Heinis, Philippe Diderich

Many naturally occurring cyclic peptides or derivatives thereof are used as therapeutics such as the human hormones vasopressin and oxytocin or the antibiotics vancomycin and daptomycin. The success of cyclic peptide therapeutics is based on their ability ...

Schweizerische Chemische Gesellschaft2013

Polycyclic Peptide Therapeutics

Christian Heinis, Vanessa Baeriswyl

Owing to their excellent binding properties, high stability, and low off-target toxicity, polycyclic peptides are an attractive molecule format for the development of therapeutics. Currently, only a handful of polycyclic peptides are used in the clinic; ex ...

Wiley-V C H Verlag Gmbh2013

Retour en ville

Face aux multiples conséquences induites par l’étalement urbain, un consensus se dégage progressivement pour réorienter le développement du bâti vers l’intérieur et pour favoriser les processus de densification urbaine. Dans ce contexte, des stratégies de ...

Presses polytechniques et universitaires romandes2013

Tracking chemical reactions on the surface of filamentous phage using mass spectrometry

Christian Heinis, Shiyu Chen, Jeremy Touati

Chemical modification of phage libraries has allowed the in vitro evolution of ligands having properties not provided by natural polypeptides. The development of novel and more diverse chemical reactions on phage was hampered by the lack of analytical meth ...

2013

Phenotypic Profiling of Mycobacterium tuberculosis EspA Point Mutants Reveals that Blockage of ESAT-6 and CFP-10 Secretion In Vitro Does Not Always Correlate with Attenuation of Virulence

Stewart Cole, Neeraj Dhar, Florence Pojer, Ming Zhang, Jan Lars Rybniker, Jeffrey Chen, Anna De Graff Tischler

The EspA protein of Mycobacterium tuberculosis is essential for the type VII ESX-1 protein secretion apparatus, which delivers the principal virulence factors ESAT-6 and CFP-10. In this study, site-directed mutagenesis of EspA was performed to elucidate it ...

American Society for Microbiology2013

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Directed evolution

Phage Selection of Photoswitchable Peptide Ligands

Fluorescent Labeling of SNAP-Tagged Proteins in Cells

Development of mutagenicity during degradation of N-nitrosamines by advanced oxidation processes

Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae

Directed Evolution of Bicyclic Peptides for Therapeutic Application

Polycyclic Peptide Therapeutics

Retour en ville

Development of New Bicyclic Peptide Formats and their Application in Directed Evolution

Tracking chemical reactions on the surface of filamentous phage using mass spectrometry

Phenotypic Profiling of Mycobacterium tuberculosis EspA Point Mutants Reveals that Blockage of ESAT-6 and CFP-10 Secretion In Vitro Does Not Always Correlate with Attenuation of Virulence

Graph Chatbot

Chat with Graph Search

Phage Selection of Photoswitchable Peptide Ligands

Directed Evolution of Bicyclic Peptides for Therapeutic Application

Fluorescent Labeling of SNAP-Tagged Proteins in Cells

Development of mutagenicity during degradation of N-nitrosamines by advanced oxidation processes

Retour en ville

Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae

Development of New Bicyclic Peptide Formats and their Application in Directed Evolution

Polycyclic Peptide Therapeutics

Tracking chemical reactions on the surface of filamentous phage using mass spectrometry

Phenotypic Profiling of Mycobacterium tuberculosis EspA Point Mutants Reveals that Blockage of ESAT-6 and CFP-10 Secretion In Vitro Does Not Always Correlate with Attenuation of Virulence