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Related publications (179)

Fine-tuning of magnetic and microfluidic viscous forces for specific magnetic bead-based immunocomplex formation

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Hüseyin Cumhur Tekin

We investigate the working principle of a novel type of microfluidic sandwich immunoassay, as used for the detection of biomarkers. The heterogeneous assay is based on the specific interactions between an array of functionalized superparamagnetic beads and ...

American Institute of Physics2013

Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation

Kai Johnsson

While antibodies currently play a dominant role as affinity reagents in biological research and for diagnostics, a broad range of recombinant proteins are emerging as promising alternative affinity reagents in detection assays and quantification. DNA-media ...

Elsevier2013

Alpha-synuclein Post-translational Modifications as Potential Biomarkers for Parkinson Disease and Other Synucleinopathies

Hilal Lashuel, Marc Moniatte, Bruno Claude Daniel Fauvet, Adrian Schmid

The development of novel therapies against neurodegenerative disorders requires the ability to detect their early, presymptomatic manifestations in order to enable treatment before irreversible cellular damage occurs. Precocious signs indicative of neurode ...

Amer Soc Biochemistry Molecular Biology Inc2013

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Gerardo Turcatti, Benjamin Rappaz, Marc Chambon, Jonas Kühn, Etienne Shaffer, Jérôme Parent, Julien Mena

Abstract We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content ...

Mary Ann Liebert2013

Micro segmented-flow in biochemical and cell-based assays

Christoph Merten

Micro-segmented flow (e.g. in microfluidic channels, capillaries or a length of tubing) has become a promising technique in modern biology. Compared to conventional formats such as microtiter plates, sample volumes can be reduced about 1000-fold, thus allo ...

2012

Automated Analysis of Rare Biomarkers Using a Magnetic Bead Surface Coverage Assay Coupled with Chaotic Mixing on a Microfluidic Chip

Hüseyin Cumhur Tekin

Extremely sensitive protein detection is crucial for early diagnosis and monitoring of diseases. Present day’s limit of detection for an antibody-antigen recognition-based immunoassay in serum without having to use special target antigen amplification tech ...

EPFL2012

Functional single-cell hybridoma screening using droplet-based microfluidics

Christoph Merten

Monoclonal antibodies can specifically bind or even inhibit drug targets and have hence become the fastest growing class of human therapeutics. Although they can be screened for binding affinities at very high throughput using systems such as phage display ...

2012

Sequencing by Hybridization of Long Targets

Tobias Schneider, Yu Qin

Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n ...

2012

Droplet-based Liquid-Liquid Extraction and On-Chip IR-Waveguide-Spectroscopy Detection of Cocaine in Human Saliva

Hans Peter Herzig, Peter Van der Wal, Lubos Hvozdara, Philip Wägli, Yu-Chi Chang

We present a portable microsystem to quantitatively detect cocaine in human saliva. The chip combines a simple microfluidic method for multiphase liquid-liquid extraction to transfer cocaine from IR-light absorbing saliva to an IRtransparent solvent with t ...

2012

Microfluidic applications of functionalized magnetic particles for environmental analysis: focus on waterborne pathogen detection

Martinus Gijs

The continuous surveillance of drinking water is extremely important to provide early warning of contamination and to ensure continuous supplies of healthy drinking water. Isolation and detection of a particular type of pathogen present at low concentratio ...

Springer-Verlag2012

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