Concept

Cell fractionation

Related publications (33)

Assessing the cellular uptake pathway for poly-Lysine analogues using triplet lifetime imaging

Dimitri Nestor Alice Van De Ville, Theo Lasser, Harm-Anton Klok, Iwan Märki, Zuzana Kadlecova

Research on synthetic delivery vectors is of major interest for cell imaging and manipulation, as they allow an efficient transfer of nucleic acids, therapeutic proteins or small drugs into the cells. We have developed a library of L-lysine analogues that ...

2011

Procentriole assembly revealed by cryo-electron tomography

Paul Guichard

Centrosomes are cellular organelles that have a major role in the spatial organisation of the microtubule network. The centrosome is comprised of two centrioles that duplicate only once during the cell cycle, generating a procentriole from each mature cent ...

2010

Molecular and biochemical aspects of the chloroethene dehalorespiration process

Christof Holliger, Julien Maillard, Aurélie Duret, Laure Prat

Tetrachloroethene (PCE) is a major groundwater pollutant as a result of extensive use and spillage since 80 years. Several obligate anaerobic bacteria have the ability of using chlorinated aliphatic compounds as terminal electron acceptor in a process call ...

2008

Comprehensive proteome analysis of Mycobacterium ulcerans and quantitative comparison of mycolactone biosynthesis

Stewart Cole, Petra Tafelmeyer

Mycobacterium ulcerans is the causative agent of Buruli ulcer, a rapidly emerging human disease in which mycolactone, a cytotoxic and immunosuppressive macrocyclic polyketide, is responsible for massive skin destruction. The genome sequencing of M. ulceran ...

2008

Micro- and nanostructured devices for investigating biomolecular interactions

Jürgen Brugger, Horst Vogel, Christian Santschi, Christophe Danelon, Jean-Baptiste Perez

Cell membrane receptors and ion channels are essential in many different cellular processes. To analyse the activity of membrane proteins in vesicles and biological cells, we fabricated micro- nanostructured chips, enabling the application of electrophysio ...

2006

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells

Florian Maria Wurm, David Hacker, Martin Bertschinger, Frédéric Grosjean

The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter g ...

2006

Salt Removal During Off-GelTM Electrophoresis of Protein Samples

Hubert Girault, Niels Erik Lion, Christophe Roussel

The Off-Gel™ technology was recently described for protein fractionation in a solution placed on top of an immobilized pH gradient gel. In addition, this process was found to remove salts from the biological samples to analyze. This desalting effect is stu ...

2005

Short-term immunosuppression reduces fibrotic cellular infiltration around barium-M-alginate microbeads injected intraportally

Philipp Bucher, Jutta Christine Wandrey

Introduction. One of the major barriers affecting the viability of encapsulated islets is pericapsular ﬁbrotic inﬁltration (PFI). This study aimed to design strategies to reduce PFI around intraportally injected alginate microbeads. Methods. Empty, highly ...

2004

Multiaperture sample positioning and analysis system

Horst Vogel

Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivs., and mixts. thereof, for elec. and/or optical anal., esp. relating to the presence and/or activity of ion channels. [on SciFinder (R)] ...

(Switz.).2003

Ligand Binding to G Protein-Coupled Receptors in Tethered Cell Membranes

Horst Vogel, Rudolf Hovius, Bruno Meyer

G protein-coupled receptors (GPCRs) constitute a large class of seven transmembrane proteins, which bind selectively agonists or antagonists with important consequences for cellular signaling and function. Comprehension of the mol. details of ligand bindin ...

2003

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