Publications related to Immunofluorescence

Super-resolution orientation estimation and localization of fluorescent dipoles using 3-D steerable filters

Michaël Unser, Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorophores that are fixed during image acquisition produce a diffraction pattern that is characteristic of the orientation of the fluorophore’s underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolu ...

OSA2009

Super-resolved position and orientation from defocused images of fluorescent dipoles

Michaël Unser, Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

2009

In vivo time-resolved spectroscopy of the human bronchial early cancer autofluorescence

Georges Wagnières, Tanja Gabrecht, Pascal Uehlinger

Time-resolved measurements of tissue autofluorescence (AF) excited at 405 nm were carried out with an optical-fiber-based spectrometer in the bronchi of 11 patients. The objectives consisted of assessing the lifetime as a new tumor/normal (T/N) tissue cont ...

2009

Exploring single-molecule dynamics with fluorescence nanoscopy

Marcel Leutenegger

The study of molecular dynamics at the single-molecule level with fluorescence correlation spectroscopy (FCS) and far-field optics has contributed greatly to the functional understanding of complex systems. Unfortunately, such studies are restricted to len ...

Institute of Physics (IoP) and Deutsche Physikalische Gesellschaft2009

Three-dimensional Nano-Localization of Fluroescent Emitters

Theo Lasser, Iwan Märki, François Aguet, Noelia Laura Bocchio

The use of fluorescence light for far-field imaging at the nanometer scale has drawn much attention worldwide. Example techniques include STED microscopy and localization microscopy (PALM, STORM). Typically, nanoscopy approaches have been focused on the ma ...

2009

Autofluorescence detection of tumors in the human lung - spectroscopical measurements in situ, in an in vivo model and in vitro

Georges Wagnières, Tanja Gabrecht

To detect bronchial carcinoma by autofluorescence, we measured the spectra of tumor and normal tissue in situ, in an in vivo model and in vitro by fiber optic spectrometer and two-dimensional resolved microspectroscopy. The in situ measurements were perfor ...

Elsevier2008

AMPA receptor imaging by infrared scanning near-field optical microscopy

Giorgio Margaritondo, Kai Johnsson, Harald Hirling, Johanna Generosi, Michel Kropf

Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 µm wavel ...

2008

Towards in vivo small animal imaging

Theo Lasser, Michael Friedrich, Rainer Leitgeb, Anne Grapin-Botton, Martin Villiger, Joan Goulley

Fourier domain Optical coherence microscopy (FDOCM) offers excellent sensitivity and high axial resolution to image the structure of biological tissue. The depth information is extracted in parallel and allows very high volume acquisition rates. An illumin ...

2008

Rapid Immunoassay Using Steady-State Dispersion Effects in Nanochannels

Philippe Renaud, Nicolas Durand, Ana Valero

We present a novel method for on-chip competitive dispersion immunoassay (DISIA) which aims at measuring the specificity between fluorescently-labeled antibodies (Ab*) and label-free antigens (Ag). We have fabricated a nanofluidic device that allows fast d ...

2008

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Immunofluorescence

Super-resolution orientation estimation and localization of fluorescent dipoles using 3-D steerable filters

Super-resolved position and orientation from defocused images of fluorescent dipoles

In vivo time-resolved spectroscopy of the human bronchial early cancer autofluorescence

Exploring single-molecule dynamics with fluorescence nanoscopy

Three-dimensional Nano-Localization of Fluroescent Emitters

Autofluorescence detection of tumors in the human lung - spectroscopical measurements in situ, in an in vivo model and in vitro

AMPA receptor imaging by infrared scanning near-field optical microscopy

Towards in vivo small animal imaging

Rapid Immunoassay Using Steady-State Dispersion Effects in Nanochannels

Quantitative single-molecule imaging by confocal laser scanning microscopy

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Chat with Graph Search

Autofluorescence detection of tumors in the human lung - spectroscopical measurements in situ, in an in vivo model and in vitro

Exploring single-molecule dynamics with fluorescence nanoscopy

Super-resolved position and orientation from defocused images of fluorescent dipoles

Super-resolution orientation estimation and localization of fluorescent dipoles using 3-D steerable filters

Three-dimensional Nano-Localization of Fluroescent Emitters

In vivo time-resolved spectroscopy of the human bronchial early cancer autofluorescence

Rapid Immunoassay Using Steady-State Dispersion Effects in Nanochannels

AMPA receptor imaging by infrared scanning near-field optical microscopy

Quantitative single-molecule imaging by confocal laser scanning microscopy

Towards in vivo small animal imaging