Concept

Expression vector

Related publications (93)

Identification of genetic insulator elements to increase the safety of viral gene therapy vectors

Haematopoeitic system diseases, both acquired and inherited, can be currently cured by allogeneic haematopoietic stem cell transplantation. This treatment provides highly successful immune function recovery for patients receiving grafts of HLA-compatible d ...

EPFL2010

Modeling Parkinson's Disease in Adult Rats Using Viral Vectors as Gene Delivery Tools

Julien Dusonchet

Animal models of human pathologies remain invaluable tools for unraveling disease mechanisms and evaluating potential therapeutic strategies. For a number of diseases, the lack of a reliable animal model represents an important limiting step towards the de ...

EPFL2010

Development of a positive and negative drug selection system towards gene correction in medaka fish haploid ES cells

This project aimed to develop an improved positive-negative selection system for potential use in basic research and clinical applications. The NTR-CB1954 system was chosen, where introduced expression of the E. Coli derived gene product nitroreductase (NT ...

2010

Analysis of the Pigment Cell Specific Gene MART-1/Melan-A/mlana

Iraz Toprak Aydin

Pigmentation is a complex process that provides many diverse benefits for the organism. Pigment production takes place in the pigment cell lineage in dedicated organelles called melanosomes. Several proteins are specifically recruited to melanosomes for th ...

EPFL2010

Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture

Florian Maria Wurm, David Hacker, Agata Oberbek

Lentivirus-derived vectors (LVs) were studied for the generation of stable recombinant Chinese hamster ovary (CHO) cell lines. Stable pools and clones expressing the enhanced green fluorescent protein (eGFP) were selected via fluorescence-activated cell so ...

Wiley-Blackwell2010

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiaowei Zhang, Isabel Fernandez Garcia

The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold rel ...

Springer Netherlands2010

Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology

Florian Maria Wurm, David Hacker, Lucia Baldi Unser

Recombinant protein-based biologics are increasingly relevant in the pharmaceutical industry. Currently, mammalian cell-based bioprocesses are a routine manufacturing procedure for complex proteins such as recombinant antibodies, and the number of new biol ...

Wiley2009

Transient recombinant protein expression in mammalian cells

Sarah Wulhfard

Transient gene expression (TGE) is a rapid method for generating recombinant proteins in mammalian cells, but the volumetric productivities for secreted proteins in transiently transfected CHO DG44 cells are typically more than an order of magnitude lower ...

EPFL2009

Imaging of G protein-coupled receptors in solid-supported planar lipid membranes

Theo Lasser, Marcel Leutenegger

This contribution summarizes first some of our efforts in imaging G-protein-coupled receptor (GPCR) functional inserted into planar tethered lipid bilayer membranes (tBLMs) as a novel platform for biophysical studies. The authors introduced recently a nove ...

2009

25 years of recombinant proteins from reactor-grown cells — Where do we go from here?

Florian Maria Wurm, David Hacker

The purpose of this review is to describe the current status and to highlight several emerging trends in the manufacture of recombinant therapeutic proteins in cultivated mammalian cells. focusing on Chinese hamster ovary cells as the major production host ...

Elsevier2009

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