Concept

2-Phosphoglycolate

2-Phosphoglycolate (chemical formula C2H2O6P3-; also known as phosphoglycolate, 2-PG, or PG) is a natural metabolic product of the oxygenase reaction mediated by the enzyme ribulose 1,5-bisphosphate carboxylase (RuBisCo). RuBisCo catalyzes the fixation of atmospheric carbon dioxide in the chloroplasts of plants. It uses ribulose 1,5-bisphosphate (RuBP) as substrate and facilitates carboxylation at the C2 carbon via an endiolate intermediate. The two three-carbon products (3-phosphoglycerate) are subsequently fed into the Calvin cycle. Atmospheric oxygen competes with this reaction. In a process called photorespiration RuBisCo can also catalyze addition of atmospheric oxygen to the C2 carbon of RuBP forming a high energy hydroperoxide intermediate that decomposes into 2-phosphoglycolate and 3-phosphoglycerate. Despite a higher energy barrier for the oxygenation reaction compared to carboxylation, photorespiration accounts for up to 25% of RuBisCo turnover in C3 plants. In plants, 2-phosphoglycolate has a potentially toxic effect as it inhibits a number of metabolic pathways. The activities of important enzymes in the central carbon metabolism of the chloroplast such as triose-phosphate isomerase, phosphofructokinase, or sedoheptulose 1,7-bisphosphate phosphatase show a significant decrease in the presence of 2-PG. Therefore, degradation of 2-PG during photorespiration is important for cellular homeostasis. Photorespiration is the main way of chloroplasts to rid themselves of 2-PG. However, this pathway comes at a decreased return on investment ratio as 2-PG is transformed to 3-phosphoglycerate in an elaborate salvage pathway at the cost of one equivalent of NADH and ATP, respectively. In addition, this salvage pathway loses 1⁄2 equivalent of previously fixed carbon dioxide and releases 1⁄2 equivalent of toxic ammonia per molecule of 2-PG. This leads to a net loss of carbon in photorespiration, making it much less efficient than the Calvin cycle.

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