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This lecture focuses on the targeting of glycolysis and glycolytic signaling switches in cancer, exploring the chemical biology toolsets and applications, enzyme inhibition mechanisms, and high-throughput proteomics screening techniques. It delves into the role of activated carriers in metabolic chemistry, the eukaryotic metabolism pathways, and the TCA cycle. The lecture also covers the essential role of ATP as the body's energy currency, the cellular respiration process, and the interconnected pathways of glycolysis, TCA cycle, and oxidative phosphorylation.