Chemical Biology Toolsets: Enzyme Inhibition and Target Modulation

This lecture covers the chemical biology toolsets related to enzyme inhibition, including reversible (competitive, non-competitive, TS analog) and irreversible mechanisms. It also discusses small-molecule-based target modulation techniques like the Bump-Hole method and ABPP indirect profiling. Additionally, it explores transcriptome/genome-probing tools such as mRNA display and RNA aptamers. The lecture delves into proteome-level probing tools like the Amber suppressor technique and SILAC-BONCAT method, highlighting their applications in chemical biology.