Chemical Biology Toolsets: Enzyme Inhibition and Metabolic Signaling

This lecture covers enzyme inhibition mechanisms of small-molecule drugs, including reversible and irreversible inhibition. It also explores small-molecule-based target modulation techniques and metabolic signaling concepts. The instructor delves into the Chemical Biology of Glycolysis, TCA cycle, and Oxidative Phosphorylation, highlighting their importance in drug discovery. Additionally, the lecture discusses the role of activated carriers in metabolic chemistry and the significance of the TCA cycle in central carbon metabolism. Various isotopic tracing methods for analyzing central carbon metabolism are introduced, emphasizing the application of LC-MS/MS for metabolic pathway analysis.