Long-read Sequencing Techniques

This lecture introduces long-read sequencing techniques based on nanopore technology, which allows for the sequencing of kilobases-length single reads without the need for ligation or elongation. The instructor explains the physical principles behind nanopore sequencing, the role of membrane proteins, and the correlation between DNA passage through a nanopore and voltage changes. The lecture also covers PacBio sequencing, which utilizes adaptors to form a circular DNA template for accurate base determination and reading longer DNA stretches.