This lecture covers the instrumentation used in electron microscopy for life sciences, including techniques like SPECT, PET, CT, MRI, OCT, synchrotron X-ray tomography, and helium microscopy. It explains the limitations of single instruments in covering all scales, the components of a transmission electron microscope, electron sources, and various electron microscopes used in research. The lecture also delves into specimen preparation, electron beam interactions, scattering terminology, and contrast transfer functions in electron microscopy.