Lecture
This lecture covers the functionality of large area architecture, focusing on optical techniques for imaging and analyzing brain structures. Topics include the generation of multilevel maps, large area imaging methods, tissue clearing procedures, and the application of various microscopy techniques. The instructor discusses the challenges and advantages of different clearing methods, such as 2,2′ Thiodiethanol (TDE), for achieving transparent brain samples and enabling detailed imaging. The lecture also explores the validation of clusters in mouse brain tissue, whole hippocampus imaging, post mortem labeling techniques, and human brain imaging using serial two-photon microscopy. Additionally, the lecture delves into multi-round antibody labeling and bridging the gap between molecular and organ-level imaging, emphasizing the compatibility of TDE with electron microscopy.