This lecture explores the advancements in optical computational imaging, focusing on overcoming the diffraction limit and imaging through complex media. Techniques like fluorescence imaging with single molecule sensitivity and three-dimensional point spread function engineering are discussed, along with imaging through strongly scattering media. The talk delves into the potential of these techniques in biological imaging, showcasing the work of the instructor, Prof. Rafael Piestun, in computational optical imaging, super-resolution microscopy, and scattering optics.