Fluorescence Lifetime in Photomedicine

This lecture covers the health effects of artificial light, fluorescence guided surgery, and the applications of optical spectroscopy in photomedicine, focusing on fluorescence lifetime measurements and quenching mechanisms. It explains the principles and methodologies of time-resolved fluorescence measurements, including pulse and frequency domain methods, as well as time-correlated single photon counting. The instructor encourages class presentations on related topics, emphasizing the importance of topic approval. Various environmental sensitive dyes for sensing applications are discussed, along with the instrument considerations for fluorescence lifetime measurements. The lecture concludes with a detailed explanation of single photon counting methodology and instrumentation components for precise fluorescence decay measurements.