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This lecture covers the principles of light dosimetry in tissues for photomedicine applications, including tissue optics, mechanisms of light/laser therapy, classification of medical lasers, accuracy of laser therapy models, and the influence of laser pulse duration on treatment outcomes. The lecture also discusses the importance of the light beam diameter, thermal relaxation time, and the selective thermal action enabled by specific parameters of laser beams. Various applications of optical spectroscopy in photomedicine, such as absorption and fluorescence spectroscopies, are explored, along with the energy levels involved in luminescence. The session concludes with an overview of the Jablonski diagram and its significance in explaining the kinetics and spectra of fluorescence, phosphorescence, and delayed fluorescence.