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This lecture explores the role of thermodynamics in cell biology, focusing on how thermodynamics governs processes from the Big Bang to cellular activities. It discusses the connection between experiments and theory, the universality of thermodynamics, classical mechanics, classical thermodynamics, idealizations in thermodynamics, and the predictions made by thermodynamics. The lecture also covers microscopic thermodynamics, a brief history of thermodynamics, the concept of equilibrium, and the types of processes. Furthermore, it delves into the ensemble, work, internal energy, the first law of thermodynamics, reversible processes, exact and inexact differentials, and the application of thermodynamics in cell biology.