Lecture
This lecture covers the structure, properties, and analysis of proteins, including amino acid physico-chemical properties, ionization states, isoelectric point, solubility, post-translational modifications, and secondary structure analysis using circular dichroism spectroscopy. It also discusses protein analysis techniques such as isoelectric focusing, 2D gel electrophoresis, and secondary structure prediction. The instructor emphasizes the importance of understanding protein charge, predicting molecular weight, and interpreting UV and CD spectra.