This lecture covers the fundamentals of protein mass spectrometry and proteomics, including the introduction to protein analysis, mass spectrometry reminders, ionization sources, analyzers, and detectors used in proteomics. It also delves into proteomic strategies, workflows, separations techniques, quantitative proteomic workflows, proteomic bioinformatics, and applications to biology and clinical research. The content includes discussions on bottom-up proteomics, peptide mass fingerprinting, protein identification using MS/MS data, and the comparison between PMF and MS/MS ion search methods.