This lecture covers the optimal choice of ultrasound (US) frequency in biomedical imaging, discussing how resolution and signal-to-noise ratio (SNR) are affected by frequency. It also explores examples of high-resolution US at the surface and low-resolution US of deep tissues, as well as 3D US imaging principles and the use of contrast agents. Additionally, it explains how ultrasound detects moving blood using the Doppler effect, with practical examples and applications.