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This lecture covers the replication of DNA in eukaryotes, focusing on the challenges faced due to the large size and linear nature of chromosomes. It explains the mechanisms used to replicate chromosome ends, such as the role of telomeres and telomerase. The process of DNA replication, including the involvement of various enzymes like DNA polymerase, DNA primase, and DNA ligase, is detailed. Additionally, the lecture discusses the importance of the shelterin complex in protecting chromosome ends and preventing DNA damage detection. Overall, it provides a comprehensive overview of the intricate process of DNA replication in eukaryotic cells.