Introduction to Flow Cytometry

This lecture provides an overview of flow cytometry, a powerful technology for analyzing thousands of cells per second and detecting multiple parameters within heterogeneous populations. The instructor, Miguel Garcia, covers the principles of flow cytometry, the components of a cytometer, the fluidics system, optics, lasers, filters, detectors, and electronic signal conversion. The lecture also delves into the applications of flow cytometry, such as immunophenotyping, cell cycle analysis, cell sorting, and analysis of rare cell populations. Additionally, it explores the concepts of fluorescence, fluorophores, and DNA analysis using flow cytometry techniques.