Introduction to Flow Cytometry

This lecture provides an overview of flow cytometry, covering topics such as the definition, components, fluidics system, lasers, filters, detectors, fluorescence, and signal measurement. It explains the principles of forward and side scatter, the flow cell, hydrodynamic focusing, and fluorescence intensity. The lecture also discusses the applications of flow cytometry, including immunophenotyping, cell cycle analysis, and cell sorting. New technologies like imaging flow cytometry and mass cytometry are introduced, along with the concept of full spectral flow cytometry. The presentation delves into the cancer immunity cycle, immunoediting theory, and cancer immunology, highlighting the role of different immune cells in tumor suppression and progression.