Proximity-specific translatome profiling

This lecture delves into the spatial regulation of ribosomal protein translation in eukaryotes, focusing on the role of the endoplasmic reticulum (ER) in protein synthesis and trafficking. It covers the architecture of a mammalian cell, the differences in transcription and translation between bacteria and animal cells, and the dynamics of translation using polysome/ribosome profiling. The lecture also explores the emerging role of the ER as a hub for protein synthesis, detailing the processes of protein targeting and sorting. Advanced concepts such as polysome fractionation, biotin-streptavidin binding, and experimental designs for analyzing translation dynamics are discussed, providing insights into the critical evaluation of research manuscripts.