Proximity-specific Ribosome Profiling

This lecture delves into the interrogation of the local translatome through proximity-specific ribosome profiling, focusing on translational dynamics with high spatiotemporal resolution. Key concepts include spatial regulation of ribosomal protein translation, the architecture of a mammalian cell, and the role of the endoplasmic reticulum in protein synthesis. The lecture also covers the translation process in bacteria versus animal cells, the distribution of translation between the cytosol and endoplasmic reticulum, and the emerging role of the endoplasmic reticulum as a hub for protein synthesis. Advanced topics such as polysome fractionation, biotin-streptavidin binding, and experimental designs for ribosome profiling are discussed.