This lecture covers the nitrogen and sulfur cycles in biogeochemistry, focusing on the oxidation of ammonia to nitrite and the subsequent oxidation of nitrite to nitrate. It explains the role of key enzymes like Ammonia Monooxygenase (AMO) and HydroxylAmine Oxidoreductase (HAO) in these processes, as well as the importance of nitrification and anammox in wastewater treatment. The lecture also delves into microbial transforming networks in various environments, such as oceans, agricultural soil, and rice paddies, highlighting the diverse range of bacteria involved in nitrogen transformations.