Proteomics Analysis: Enzymes, Workflows, and Applications

This lecture covers the analysis of peptides in proteomics, including the use of enzymes like trypsin, Lys-C, and chymotrypsin for protein digestion. It explains workflows such as top-down and bottom-up approaches, as well as techniques like Peptide Mass Fingerprinting (PMF) and Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS). The importance of data-dependent acquisition (DDA) and the role of different enzymes in protein/peptide enrichment are also discussed.