Lecture

Proteomics Analysis: Enzymes, Workflows, and Applications

In course

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Description

This lecture covers the analysis of peptides in proteomics, including the use of enzymes like trypsin, Lys-C, and chymotrypsin for protein digestion. It explains workflows such as top-down and bottom-up approaches, as well as techniques like Peptide Mass Fingerprinting (PMF) and Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS). The importance of data-dependent acquisition (DDA) and the role of different enzymes in protein/peptide enrichment are also discussed.

Instructor

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Official source

https://mediaspace.epfl.ch/media/0_nurm122n

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Ontological neighbourhood

Biology

Genetics: Genomics

Chemistry

Analytical chemistry: Mass spectrometry

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