Small-Molecule Therapeutics and Protein Interactions

This lecture discusses the use of small-molecule therapeutics to perturb cell signaling and druggable interactions. It covers functionalizing ligands with an alkyne unit, performing Click coupling reactions, and analyzing samples via gel electrophoresis. The lecture also explores identifying anti-allergic properties in molecules, reacting functional groups with amino-acid side chains, and studying protein bands over time. Additionally, it delves into targeting disease-specific mutant proteins and making adaptations to ligands. Experimental data from purified proteins and cell lysates are presented and analyzed.