Lecture
This lecture explores circular permutation in protein engineering, a technique used to optimize proteins by introducing new N- and C-termini without altering the protein structure. The instructor discusses the concept of circular permutation, the process of creating new termini, and the importance of linkers in maintaining protein functionality. Additionally, the lecture covers the review of FRET parameters and the application of circular permutation in FRET optimization. Examples of circular permutation in genetically encoded fluorescent indicators and voltage biosensors are also presented.