This lecture introduces the general aspects of microscopy, including magnification, resolution, contrast, and aberrations. It covers commonly used microscopy techniques in biology such as electron microscopy, scanning probe microscopy, and light microscopy. The importance of resolution in microscopy is emphasized, along with the different types of microscopes and their applications. The lecture also delves into the principles behind scanning tunneling microscopy and atomic force microscopy, highlighting their significance in imaging at the atomic and molecular levels.