This lecture focuses on the transformation of GAP to DPG in the Krebs cycle, involving the cofactor NADP and the production of H+ and H2O. The instructor explains the concept of endergonic and exergonic reactions, calculates the standard free energy change, and discusses how to shift the reaction equilibrium by manipulating concentrations. By analyzing the dynamic equilibrium in biochemical reactions, the lecture provides insights into the interplay of different molecules and the importance of real-time conditions over standard conditions.