Protein Expression and Purification

This lecture covers the process of expressing and purifying proteins, including the importance of studying protein function, folding, interactions, sequence, and structure. It discusses the use of different hosts for protein production, the anatomy of a bacterial expression plasmid, and the steps involved in protein purification. The lecture also explains the goals of protein purification, various cell lysis methods, chromatography buffers, and methods for detecting the protein of interest.