Protein Expression & Purification

This lecture covers the process of expressing and purifying proteins, focusing on gel filtration to isolate protein complexes. Topics include recombinant protein expression, host selection, cell lysis methods, chromatography buffers, detection methods, and affinity purification using tags like 6xHis. The instructor explains the importance of protein purification, the use of different hosts for expression, and the steps involved in recombinant protein purification. The lecture also discusses the principles of chromatography, including ion-exchange and size-exclusion chromatography, and the use of cleavage sites for tag removal.