Publications by François Aguet

3-D PSF fitting for fluorescence microscopy: implementation and localization application

Michaël Unser, Daniel Sage, François Aguet, Hagay Kirshner

Localization microscopy relies on computationally efficient Gaussian approximations of the point spread function for the calculation of fluorophore positions. Theoretical predictions show that under specific experimental conditions, localization accuracy i ...

Wiley-Blackwell2013

Rotational Features Extraction for Ridge Detection

Michaël Unser, Pascal Fua, François Fleuret, François Aguet, Germán González Serrano, Fethallah Benmansour

State-of-the-art approaches to detecting ridge-like structures in images rely on filters designed to respond to locally linear intensity features. While these approaches may be optimal for ridges whose appearance is close to being ideal, their performance ...

Institute of Electrical and Electronics Engineers2011

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets of fluorophores, which are localized by means of Gaussian-based localization. Applied to fixed fluorophores, with dipole ...

2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Optical Imaging Using Binary Sensors

Michaël Unser, François Aguet, Aurélien Bourquard

This paper addresses the problem of reconstructing an image from 1-bit-quantized measurements, considering a simple but nonconventional optical acquisition model. Following a compressed-sensing design, a known pseudo-random phase-shifting mask is introduce ...

OSA2010

Three-dimensional Nano-Localization of Fluroescent Emitters

Theo Lasser, Iwan Märki, François Aguet, Noelia Laura Bocchio

The use of fluorescence light for far-field imaging at the nanometer scale has drawn much attention worldwide. Example techniques include STED microscopy and localization microscopy (PALM, STORM). Typically, nanoscopy approaches have been focused on the ma ...

2009

Three-dimensional Localization of Fluorescent Emitters at the Nano-scale

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

We demonstrate nanometer-level localization accuracy of a single fluorescent emitter in three dimensions. Our super resolution microscopy technique is based on spectral self-interference for axial localization and two-dimensional diffraction pattern analys ...

2009

Steerable filters for orientation estimation and localization of fluorescent dipoles

Michaël Unser, Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy (i.e., PALM, STORM) has enabled optical imaging at nanometer-scale resolutions. The localization algorithms used in these techniques rely on fitting a 2-D Gaussian to the in-focus image of individual fluorophores. For f ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2009

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François Aguet

3-D PSF fitting for fluorescence microscopy: implementation and localization application

Rotational Features Extraction for Ridge Detection

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Three-dimensional nano-localization of single fluorescent emitters

Optical Imaging Using Binary Sensors

Super-resolution fluorescence microscopy based on physical models

Three-dimensional Nano-Localization of Fluroescent Emitters

Three-dimensional Localization of Fluorescent Emitters at the Nano-scale

Steerable filters for orientation estimation and localization of fluorescent dipoles

Graph Chatbot

Chat with Graph Search

Three-dimensional nano-localization of single fluorescent emitters

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Optical Imaging Using Binary Sensors

Rotational Features Extraction for Ridge Detection

Super-resolution fluorescence microscopy based on physical models

Three-dimensional Nano-Localization of Fluroescent Emitters

Three-dimensional Localization of Fluorescent Emitters at the Nano-scale

Steerable filters for orientation estimation and localization of fluorescent dipoles

3-D PSF fitting for fluorescence microscopy: implementation and localization application

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters