Publications by Martin Villiger

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Arno Pino Bouwens, Corinne Berclaz, Daniel Pawel Szlag, Jérôme Extermann, Joan Goulley

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Diabetes imaging — quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy

Dimitri Nestor Alice Van De Ville, Theo Lasser, Anthony Christopher Davison, Anne Grapin-Botton, Martin Villiger, Arno Pino Bouwens, Corinne Berclaz, Joan Goulley, Erica Martin-Williams

Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Cohere ...

2012

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Theo Lasser, Christian Santschi, Martin Villiger, Christophe Pache, Arno Pino Bouwens, Corinne Berclaz, Noelia Laura Bocchio, Matthew Gibson, Joan Goulley

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed ...

Optical Society of America2012

Label-free imaging of cerebral β-amyloidosis with extended-focus optical coherence microscopy

Theo Lasser, Patrick Fraering, Martin Villiger, Christophe Pache, Arno Pino Bouwens, Tristan Bolmont, Mitko Dimitrov, Corinne Berclaz

We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amylo ...

Society for Neuroscience2012

Dark field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

The invention relates to an optical coherence microscopy system for fast, phase resolved imaging by means of optical coherence microscopy with decoupled illumination and detection apertures, producing a dark-field effect with an enhanced optical contrast. ...

2011

Functional optical coherent imaging platform for medical application e.g. neurosurgery, has active camera unit that captures image of specific region of test subject after transmitting stimulus signals

Theo Lasser, Dimitri Nestor Alice Van De Ville, Marcel Leutenegger, Martin Villiger, Iwan Märki, Erica Martin-Williams, Michael Friedrich

The present invention relates to a functional optical coherent imaging (fOCI) platform comprising at least one active camera unit (ACU) comprising a coherent and/or a partially coherent light source, and means for spectral filtering and imaging a selected ...

2010

Dark-field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light origina ...

Optical Society of America2010

Image formation and tomogram reconstruction in optical coherence microscopy

Theo Lasser, Martin Villiger

In this work we present a model for image formation in optical coherence microscopy. In the spectral domain detection, each wavenumber has a specific coherent transfer function that samples a different part of the object's spatial frequency spectrum. The r ...

2010

Extended Focus Optical Coherence Microscopy : Theory and Application

Martin Villiger

Optical coherence tomography (OCT) is an interferometric imaging technique that can provide depth resolved cross-sectional views of biological tissue. OCT employs light with low temporal and yet high spatial coherence. The sample is illuminated point by po ...

EPFL2010

Towards high resolution optical imaging of beta cells in vivo

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Erica Martin-Williams, Joan Goulley

Endocrine beta cells produce and release insulin in order to tightly regulate glucose homeostasis and prevent metabolic pathologies such as Diabetes Mellitus. Optical imaging has contributed greatly to our current understanding of beta cell structure and f ...

Bentham Science Publishers2010