Person
Benjamin Rappaz
Related publications (38)
Automated Dual-Mode Cell Monitoring To Simultaneously Explore Calcium Dynamics and Contraction-Relaxation Kinetics within Drug-Treated Stem Cell-Derived Cardiomyocytes
Gerardo Turcatti, Benjamin Rappaz, Bokyoung Kim, Pierre Marquet
This manuscript proposes a new dual-mode cell imaging system for studying the relationships between calcium dynamics and the contractility process of cardiomyocytes derived from human-induced pluripotent stem cells. Practically, this dual-mode cell imaging ...
AMER CHEMICAL SOC2023Image- and Fluorescence-Based Test Shows Oxidant-Dependent Damages in Red Blood Cells and Enables Screening of Potential Protective Molecules
Gerardo Turcatti, Benjamin Rappaz, David Crettaz, Michel Prudent, Manon Sandra Bardyn
An increase of oxygen saturation within blood bags and metabolic dysregulation occur during storage of red blood cells (RBCs). It leads to the gradual exhaustion of RBC antioxidant protective system and, consequently, to a deleterious state of oxidative s ...
2021Marker-Free Automatic Quantification of Drug-Treated Cardiomyocytes with Digital Holographic Imaging
Gerardo Turcatti, Benjamin Rappaz, Fabien Kuttler, Bokyoung Kim, Pierre Marquet
We use quantitative phase digital holographic microscopy (QP-DHM) to image and quantify the beating movement of cardiomyocytes, derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. The development of an analysis algo ...
AMER CHEMICAL SOC2020Image-Based Marker-Free Screening of GABA(A) Agonists, Antagonists, and Modulators
Gerardo Turcatti, Benjamin Rappaz, Fabien Kuttler, Damiano Banfi, Pierre Marquet, Pascal Jourdain
The ionotropic GABA(A) receptors represent the main target for different groups of widely used drugs having hypnotic and anxiolytic effects. So far, most approaches used to assess GABA activity involve invasive low -throughput electrophysiological techniqu ...
2019Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging
Gerardo Turcatti, Benjamin Rappaz, Pierre Marquet, Michel Prudent, Manon Sandra Bardyn
We propose methods to quantitatively calculate the fluctuation rate of red blood cells with nanometric axial and millisecond temporal sensitivity at the single-cell level by using time-lapse holographic cell imaging. For this quantitative analysis, cell me ...
OPTICAL SOC AMER2018Red blood cells ageing markers: a multi-parametric analysis
Gerardo Turcatti, Niels Erik Lion, Benjamin Rappaz, David Crettaz, Michel Prudent, Manon Sandra Bardyn
Background. Red blood cells collected in citrate-phosphate-dextrose can be stored for up to 42 days at 4 degrees C in saline-adenine-glucose-mannitol additive solution. During this controlled, but nevertheless artificial, ex vivo ageing, red blood cells ac ...
Simti Servizi Srl2017Automated tracking of temporal displacements of a red blood cell obtained by time-lapse digital holographic microscopy
Red blood cell (RBC) phase images that are numerically reconstructed by digital holographic microscopy (DHM) can describe the cell structure and dynamics information beneficial for a quantitative analysis of RBCs. However, RBCs investigated with time-lapse ...
Optical Soc Amer2016Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases
Pierre Magistretti, Christian Depeursinge, Benjamin Rappaz, Pierre Marquet, Pascal Jourdain, Kaspar Rothenfusser
Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation ...
Spie-Int Soc Optical Engineering2016Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target Screening
Gerardo Turcatti, Benjamin Rappaz, Fabien Kuttler
Cellular assays using label-free Digital Holographic Microscopy (DHM) have been previously validated for cell viability assays in a drug screening context. Our automated DHM system allows performing fast and cost-effective screening assays for a wide range ...
Springer2015Automated multi-parameter measurement of cardiomyocytes dynamics with digital holographic microscopy
Gerardo Turcatti, Benjamin Rappaz, Pierre Marquet
Compounds tested during drug development may have adverse effects on the heart; therefore all new chemical entities have to undergo extensive preclinical assessment for cardiac liability. Conventional intensity-based imaging techniques are not robust enoug ...
Optical Society of America2015