Publications by Hüseyin Cumhur Tekin

Dose-response curve of a microfluidic magnetic bead-based surface coverage sandwich assay

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hüseyin Cumhur Tekin

Magnetic micro-and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quant ...

Elsevier2015

Microfluidic chip for monitoring Ca2+ transport through a confluent layer of intestinal cells

Martinus Gijs, Paolo Silacci, Hüseyin Cumhur Tekin, Josias Basil Wacker, Christine Ruffert, Guy Vergères

The absorption of dietary calcium through the intestinal barrier is essential for maintaining health in general and especially of the bone system. We propose a microfluidic model that studies free calcium (Ca2+) transport through a confluent monolayer of C ...

Royal Soc Chemistry2014

Magnetic Particle-Scanning for Ultrasensitive Immunodetection On-Chip

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hüseyin Cumhur Tekin

We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ag's) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive sto ...

American Chemical Society2014

A random-walk based model to explain ultrasensitive magnetic bead-based immunoassays

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hüseyin Cumhur Tekin

We introduce a new modeling approach for understanding the fundamental principle of bead-based surface coverage immunoassays that rely on the specific interaction between surface-bound “small” magnetic beads (1 μm in diameter) and a flow of “large” magneti ...

2014

Fine-tuning of magnetic and microfluidic viscous forces for specific magnetic bead-based immunocomplex formation

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Hüseyin Cumhur Tekin

We investigate the working principle of a novel type of microfluidic sandwich immunoassay, as used for the detection of biomarkers. The heterogeneous assay is based on the specific interactions between an array of functionalized superparamagnetic beads and ...

American Institute of Physics2013

Self-assembled melamine microlens arrays for immunofluorescence enhancement

Martinus Gijs, Abdeljalil Sayah, Hüseyin Cumhur Tekin, Hui Yang

Self-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immun ...

2013

Ultrasensitive protein detection: a case for microfluidic magnetic bead-based assays

Martinus Gijs, Hüseyin Cumhur Tekin

Royal Society of Chemistry2013

Attomolar protein detection using a magnetic bead surface coverage assay

Martinus Gijs, Matteo Cornaglia, Hüseyin Cumhur Tekin

We demonstrate a microfluidic method for ultra-sensitive protein detection in serum. First, ‘large’ (2.8 μm) antibody-functionalized magnetic beads specifically capture antigen from a serum matrix under active microfluidic mixing. Subsequently, the large b ...

Royal Society of Chemistry2013

Automated Analysis of Rare Biomarkers Using a Magnetic Bead Surface Coverage Assay Coupled with Chaotic Mixing on a Microfluidic Chip

Hüseyin Cumhur Tekin

Extremely sensitive protein detection is crucial for early diagnosis and monitoring of diseases. Present day’s limit of detection for an antibody-antigen recognition-based immunoassay in serum without having to use special target antigen amplification tech ...

EPFL2012

Microchannel-guided capture antibody patterning on beads for multiple protein detection arrays

Martinus Gijs, Hüseyin Cumhur Tekin, Venkataragavalu Sivagnanam, Sébastien Vaneberg

We present the rapid fabrication of custom protein micro-arrays, in less than 35 minutes, on the surface of immobilized beads using microchannel-guided flows driven by a single syringe pump. The commercially available beads provide robust micro-array surfa ...

2011