Timothé Laforest

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Human retinal pigment epithelium cells can be imaged in vivo with a novel adaptive optics camera using transscleral illumination

Laura Emmanuelle Kowalczuk, Mathieu Künzi, Timothé Laforest, Christophe Moser

ASSOC RESEARCH VISION OPHTHALMOLOGY INC2022

Lock-in amplified differential phase contrast

Chiara Bonati, Timothé Laforest, Damien Claude-Marie Loterie, Christophe Moser

We introduce a lock-in method to increase the phase contrast in incoherent Differential Phase Contrast (DPC) imaging. The use of a smart pixel detector with in-pixel signal demodulation, paired with synchronized illumination, provides the basis of a bit-efficient approach to DPC. The experiments show an increased sensitivity by a factor of 8, for equivalent standard DPC measurements; single-shot sensitivity of 0.7 mrad at a frame rate of 1400 fps is demonstrated. This new approach may open the way for the use of incoherent phase microscopy in biological applications where extreme phase sensitivity and millisecond response time is required.

SPIE-INT SOC OPTICAL ENGINEERING2022

Lock-in incoherent differential phase contrast imaging

Chiara Bonati, Timothé Laforest, Damien Claude-Marie Loterie, Christophe Moser

We introduce a lock-in method to increase the phase contrast in incoherent differential phase contrast (DPC) imaging. This method improves the phase sensitivity by the analog removal of the background. The use of a smart pixel detector with in-pixel signal demodulation, paired with synchronized switching illumination, provides the basis of a bit-efficient approach to emulate a lock-in DPC. The experiments show an increased sensitivity by a factor of up to 8, as expected from theory, and a reduction of collected data by a factor of 70, for equivalent standard DPC measurements; single-shot sensitivity of 0.7 mrad at a frame rate of 1400 frames per second is demonstrated. This new approach may open the way for the use of incoherent phase microscopy in biological applications where extreme phase sensitivity and millisecond response time are required. (C) 2021 Chinese Laser Press

CHINESE LASER PRESS2022