Publications by Stefan Geissbuehler

Optical prism for creating multiple views of an image

Theo Lasser, Marcel Leutenegger, Stefan Geissbuehler

Optical prism comprising two external parallel and reflecting surfaces, one internal interface, parallel to said surfaces and acting as light splitter; said prism furthermore comprising an entrance side forming an angle β with respect to said interface and ...

2016

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Theo Lasser, Françoise Gisou van der Goot Grunberg, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Patrick Alain Sandoz, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

Nature Research2014

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Theo Lasser, Marcel Leutenegger, Corinne Berclaz, Noelia Laura Bocchio, Claudio Dellagiacoma, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

2012

Nonlinear Correlation Spectroscopy (NLCS)

Theo Lasser, Marcel Leutenegger, Iwan Märki, Jean-Pierre Wolf, Noelia Laura Bocchio, Luigi Bonacina, Stefan Geissbuehler

We present a novel concept for optical spectroscopy called nonlinear correlation spectroscopy (NLCS). NLCS analyses coherent field fluctuations of the second and third harmonic light generated by diffusing nanoparticles. Particles based on noncentrosymmetr ...

American Chemical Society2012

Cumulant microscopy

Theo Lasser, Claudio Dellagiacoma, Stefan Geissbuehler

The invention describes a method and a microscopy system for imaging and analysing stochastically and independently blinking point-like emitters. A multiple-order cumulants analysis in conjunction with an established blinking model enables the extraction o ...

2012