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Stefan Geissbuehler

Publications associées (8)

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Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction fluorescence microscopy of living cells. By analyzing the acquired image sequence with an advanced correlation method, i.e. a high-order cross-cumulant analysis, supe ...

Public Library of Science2016

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Optical prism comprising two external parallel and reflecting surfaces, one internal interface, parallel to said surfaces and acting as light splitter; said prism furthermore comprising an entrance side forming an angle β with respect to said interface and ...

2016

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Theo Lasser, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Patrick Alain Sandoz, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

2014

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By switching fluorophores on and off in either a deterministic or a stochastic manner, superresolution microscopy has enabled the imaging of biological structures at resolutions well beyond the diffraction limit. Superresolution optical fluctuation imaging ...

2013

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Theo Lasser, Marcel Leutenegger, Noelia Laura Bocchio, Corinne Berclaz, Claudio Dellagiacoma, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

2012

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The invention describes a method and a microscopy system for imaging and analysing stochastically and independently blinking point-like emitters. A multiple-order cumulants analysis in conjunction with an established blinking model enables the extraction o ...

2012

Nonlinear Correlation Spectroscopy (NLCS)

Theo Lasser, Marcel Leutenegger, Iwan Märki, Jean-Pierre Wolf, Noelia Laura Bocchio, Luigi Bonacina, Stefan Geissbuehler

We present a novel concept for optical spectroscopy called nonlinear correlation spectroscopy (NLCS). NLCS analyses coherent field fluctuations of the second and third harmonic light generated by diffusing nanoparticles. Particles based on noncentrosymmetr ...

American Chemical Society2012

Nonlinear Correlation Spectroscopy (NLCS) Theory

Theo Lasser, Stefan Geissbuehler

These theory files (Mathematica Notebooks) are a complement to the Thesis of M. Geissbuehler. The derivation is too lengthy to be included in this form in the thesis. For further explenations of the derivation please refer to the thesis of M. Geissbuehler ...

Ecole Polytechnique Fédérale de Lausanne (EPFL)2011