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Aliphatic and ethylene glycol esters of 5-aminolevulinic acid (ALA) are very efficient precursors of the photosensitizer protoporphyrin IX (PpIX) for photodynamic therapy; however, they diffuse passively across the cell membrane and thus lack cell selectiv ...
We synthesized molecules containing one or two dendritic segments and a rigid-rod-like segment with their structures in the solid state. The molecules with rod-dendron or dendron-rod-dendron architecture had biphenyl ester rigid segments and 3,4,5 tris(re- ...
In this thesis new imaging approaches for optical microscopy are proposed and studied. They are based on the use of dynamic structured illumination in combination with a demodulation detection concept employing CMOS image detectors. Two particular implemen ...
We investigated the condensation of calf thymus DNA by amphiphilic polystyrenem-b-poly(l-lysine)n block copolymers ( PS m -b- PLys n , m, n = degree of polymerization), using small-angle X-ray scattering, polarized optical microscopy and laser scanning con ...
This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines ...
To analyze the effects of decellularization on the biomechanical properties of porcine common carotid arteries, decellularization was performed by a detergent-enzymatic procedure that preserves extracellular matrix scaffold. Internal diameter, external dia ...
A method for fabricating sub-micrometer size adhesion sites for future experiments in cell biology is presented. Glass substrates were coated with a thin layer of InSnO and SiO2. The SiO2 was structured by means of electron beam lithography and reactive io ...
In view of future generations of biosensors, the immobilization of biomolecules onto selected materials with a well controlled topical addressability is of primary importance. Photoimmobilization is a technique allowing the immobilization of a biomolecule ...
Bioresorbable scaffolds made Of poly(L-lactic acid) (PLA) obtained by supercritical gas foaming were recently described as suitable for tissue engineering, portraying biocompatibility with primary osteoblasts in vitro and interesting mechanical properties ...
In this study a novel approach for the coating and functionalization of substrates for cell culture and tissue engineering is presented. Glass, silicon, and titanium panes were coated with an ultrathin film (30 ± 5 nm) of reactive star-shaped poly(ethylene ...